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High Resolution Melt (HRM) analysis is a powerful technique for the
analysis of mutations, polymorphisms and epigenetic differences in
double-stranded DNA samples.
qPCRBIO HRM Mix uses the novel SyGreen 2 intercalating dye. SyGreen 2
does not inhibit PCR and is a saturating, 3rd generation dye essential
for accurate SNP genotyping. Unlike first generation dyes, SyGreen 2 can
be used at high concentrations and does not have a different affinity
for AT or GC rich sequences.
Combining the latest advancements in polymerase technology and advanced
buffer chemistry we offer market leading performance with minimal or no
optimisation. qPCRBIO HRM Mix delivers accurate genotyping, even on a
class IV SNP.
Many modern real-time PCR instruments have a fast cycling mode. The
same developments that improve the sensitivity and consistency of
qPCRBIO HRM Mix in standard cycling conditions also allow for industry
leading performance in fast and ultra fast cycling conditions.
Features
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Accurate SNP genotyping
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Gene scanning
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CpG methylayion analysis
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3rd generation, saturating dye SyGreen 2
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Efficient specific amplification from GC rich and AT rich sequences
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